Protective effect and molecular mechanism of Pudilan anti-inflammatory oral liquid on lung injury in mice infected with the influenza A virus
Objective:To investigate the protective effect and molecular mechanism of Pudilan anti-inflamma-tory oral liquid on lung injury in mice infected with the influenza A virus(IAV).Methods:Forty male C57BU 6J mice were randomly divided into four groups,with 10 mice in each group:control group,model group,low-dose and high-dose Pudilan anti-inflammatory oral liquid group.The model group was created by intranasal instillation of the H1N1/PR8 virus.The Pudilan intervention groups received oral administration for 5 consecu-tive days after modeling.Results:Pudilan anti-inflammatory oral liquid can reduce lung inflammation and tis-sue damage caused by IAV infection.It can reduce the percentage of neutrophils and platelet count in the mice infected with IAV,and increase the percentage of lymphocytes(all P＜0.05);and maintain the morpholo-gy of bronchial epithelial cells.Transcriptome analysis revealed that Pudilan has a reversing effect on the ex-pression changes of genes related to the cell cytoskeleton,cell junctions,extracellular matrix,and epithelial cell secretion following influenza virus infection.Through fluorescence quantitative RT-PCR verification,it was found that the high-dose group has a regulatory effect on the gene expression of Cldn3,Col4a3,and Mybpc2(all P＜0.05).Conclusion:Pudilan may regulate the immune inflammatory response,maintain gene expression in lung epithelial cells,improve epithelial barrier integrity,and alleviate lung injury caused by influenza virus infection in mice.
Pudilan anti-inflammatory oral liquidinfluenza A viruspneumoniapulmonary epithelial barriercell junctionsmice
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