Screening and identification of plant growth-promoting rhizobacteria F13 and its effect on growth promotion and disease resistance of cowpea
In order to obtain a plant growth-promoting rhizobacteria with dual effects of medicine and fertilizer,a bacteria strain,named as F13,which has the function of dissolving phosphorus and potassium,was isolated from the grape rhizosphere soil.The bacterium was identified as Pseudomonas aeruginosa by morphological observation,physiology and biochemistry and 16S rDNA identification.The plate confrontation method determined that the fermentation broth of strain F13 had an antagonistic effect on 8 kinds of plant pathogens,and the antibacterial rate was between 63.26%and 81.53%.In the field application,it was found that the root irrigation of the strain F13 had a significant effect on the growth of cowpea.The experimental group was 26.7%,24.4%,and 11.9%,respectively,higher than that of the control group in terms of plant height,number of leaves,and stem diameter,and the yield increased by 39.68%.Compared with the control group,the experimental group increased phosphorus and potassium by 11.5%and 46.8%,and increased vitamin C and soluble protein content by 22.15%and 55.55%,respectively.In addition,strain F13 had a good control effect on cowpea powdery mildew,and the control efficacy in field reached 71.92%.Therefore,strain F13 is a rhizosphere growth-promoting bacterium with great potential for application of medicine and fertilizer.
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