红树植物桐花树内生真菌Talaromyces amestolkiae 30的次生代谢产物
The metabolites from mangrove endophytic fungus Talaromyces amestolkiae 30
刘洪亮 1赵飞 1唐凤婷 1李锦俊 1张轩 1杜志云 1黄华容 1佘志刚2
作者信息
- 1. 广东工业大学生物医药学院,广东 广州 510006
- 2. 中山大学化学学院,广东 广州 510006
- 折叠
摘要
对红树植物桐花树内生真菌Talaromyces amestolkiae 30的次级代谢产物进行了研究.采用大米固体发酵培养,色谱分离技术纯化单体,ESIMS和NMR等波谱数据分析,鉴定了12个异香豆素单体化合物:aspergillumarin A(1)、aspergillumarin B(2)、5,6-dihydroxy-3-(4-hydroxypentyl)-isochroman-1-one(3)、mucoriso-coumarin A(4)、peniisocoumarin H(5)、peniisocoumarin E(6)、dichlorodiaportin(7)、mucorisocoumarin C(8)、peniiso-coumarin G(9)、talumarin A(10)、5,6,8-trihydroxy-4-(1'-hydroxyethyl)-isocoumarin(11)和sescandelin(12),其中化合物4、6、7首次从篮状属真菌中分离得到.二倍稀释法抑菌活性测试显示,化合物4、6、7有抑制金黄色葡萄球菌作用;MTT法测试细胞毒活性,表明化合物7对前列腺癌PC-3细胞和VCaP细胞有细胞毒活性.
Abstract
The metabolites of the endophytic fungus Talaromyces amestolkiae 30 from the mangrove plant Aegiceras corniculatum(L.)Blanco were investigated.The fungus was cultured in rice medium,the monomeric compounds were isolated and purified by the chromatographic technique,and the structures of the compounds were identified by analysis of spectroscopy such as ESIMS and NMR.Twelve known analogues of isocoumarins(1-12)were isolated and identified as aspergillumarin A(1),aspergillumarin B(2),5,6-dihydroxy-3-(4-hydroxypentyl)-isochroman-1-one(3),mucorisocoumarin A(4),peniisocoumarin H(5),peniisocoumarin E(6),dichlorodiaportin(7),mucorisocoumarin C(8),peni-isocoumarin G(9),talumarin A(10),5,6,8-trihydroxy-4-(1'-hydroxyethyl)-isocoumarin(11)and sescan-delin(12).Among them,compounds 4,6 and 7 were obtained from the genus Talaromyces for the first time.The antibacterial activities of these compounds were tested in vitro using the twofold dilution method.Compounds 4,6,and 7 showed inhibitory activity against Staphylococcus aureus.The cytotoxic activity was tested by the MTT assay.Compound 7 showed cytotoxicity against prostate cancer PC-3 cells and VCaP cells.
关键词
红树林真菌/篮状菌/次级代谢产物/异香豆素类Key words
mangrove fungus/Talaromyces amestolkiae/secondary metabolites/isocoumarin引用本文复制引用
基金项目
广东省海洋经济发展(海洋六大产业)专项(粤自然资合[2021]48号)
出版年
2024
国家科技期刊平台
NSTL
万方数据