Investigating the Response of γ-Aminobutyric Acid and Its Alleviating Effects on Cadmium Stress in Rice
To explore the role of γ-aminobutyric acid(GABA)in mitigating cadmium(Cd)stress in rice,this study a-ssessed the growth of rice seedlings under Cd stress,measured plant Cd and GABA levels,analyzed the expression of key genes in the GABA synthesis pathway,and observed changes in plant height following exogenous application of GABA.The experiments were conducted using the japonica rice cultivar'Zhonghua 11'as the test material.The results demonstrate that treatments with 0.5,5,25,and 50 μmol/L of Cd all inhibited the growth of rice,with an increasing concentration lea-ding to a gradual increase in Cd accumulation in both roots and shoots.The ratio of GABA content between shoots and roots decreased gradually with increasing Cd concentration.Gene expression analysis revealed that one betaine aldehyde dehydro-genase coding gene in the roots was upregulated by 1.83-fold under Cd treatment,while the expression of three arginine decarboxylase coding genes in the roots was upregulated by more than 4.00-fold under Cd treatment.These genes likely play pivotal roles in regulating GABA synthesis under Cd stress conditions.Moreover,exogenous application of GABA re-sulted in a significant increase in GABA accumulation in rice plants.Remarkably,GABA treatment at optimal concentra-tions effectively mitigated Cd stress in rice,highlighting its potential as a promising strategy for alleviating Cd-induced tox-icity.These findings offer a reference for conducting a more comprehensive study on the role of GABA in Cd stress.
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