Under different irrigation and nitrogen levels, wild rice (Oryza longistaminata) with allelopathic potential, and Oryza sativa (RD23) without allelopathic potential and their F1 (RD23 × O.longistaminata) were used to study their allelopathy and weed-suppression effects to barnyard grass.During 20–50 days after transplanting, four levels of irrigation treatment, and three levels of nitrogen were conducted in the field.In bioassay, the allelopathic effect of extracting solution from rice leaves on barnyard grass germination was observed.The results showed that O.longistaminata gave the strongest allellopathy under the conditions of deficiency water and low nitrogen, the inhibition rates for the root length and dry weight of barnyard grass were 69.3% and 74.6%, and decreased with elongating submerging time and increasing nitrogen.The density and biomass of barnyard grass were investigated after growing together with rice 30 days in the field.Wild rice showed the best weed-suppression effects under the alternation of wet and dry in the field.After dry cultivating for wild rice, irrigation could significantly improve its control effect on barnyard grass.Furthermore, interactive effects between irrigation and nitrogen were significant on the allelopathy and weed-suppression in the field for wild rice and the F1.