Establishment of DNA Fingerprintings of Kenaf (Hibiscus cannabinus L.) Germplasm Resources with ISSR Molecular Markers
Kenaf (Hibiscus cannabinus L.) is an important economic crop in China.To identify germplasm resources and establish the data base of DNA fingerprintings, we adopted six kenaf germplasm resources to screen 80 ISSR primers, 20 of which were polymorphic.Then 20 ISSR primers were used to amplify 84 kenaf germplasm resources introduced and preserved from home and abroad.Totally 230 bands were produced, the average number of DNA bands amplified by each primer was 11.5, and the number of polymorphic DNA bands was 185.The polymorphic proportion of DNA bands was 80.43％, which indicated the abundant genetic diversity of kenaf germplasm resources preserved.Based on the DNA bands amplified from 84 kenaf germplasm resources, the Excel data base for PCR amplified bands of the kenaf germplasm resources was established.According to the principle of uniqueness of the fingerprintings, the DNA fingerprints of 82 kenaf germplasm were constructed with five ISSR primers (UBC813, UBC825, UBC836, UBC888, and UBC889) selected from 20 polymorphic primers, using the analyzing software designed by our laboratory programmed and based on image manipulation.The fingerprintings provide a basis of the molecular identification of kenaf germplasm resources.In this research, the DNA fingerprintings system was proved to be feasible and reliable.
国家科技期刊平台
NSTL
万方数据
维普
