DNA Fingerprinting of Sesame (Sesamum indicum L.) Varieties (Lines) from Recent National Regional Trials in China
DNA fingerprinting is an important technique for plant variety identification and protection. In this study, 43 sesame (Sesamum indicum L.) varieties (lines) from recent National Regional Trails were amplified with 12 pairs of SSR primers, and a total of 30 polymorphic bands were obtained. These molecular markers were further used for variety (lines) distinction and uniformity analysis. Clustering analysis showed that 43 varieties (lines) were completely differed from each other with 30 markers. With a genetic similarity coefficient threshold of 0.90, 90% of varieties (lines) were distinctive, and 80% of tested varieties (lines) were classified into high uniformity group in consistence with marker loci uniformity, Furthermore, in assignment test, 86% of derivative individuals were correctly corresponding to their original varieties (lines), it indicated that most varieties (lines) have a good uniformity. Overall, the results showed that most of the tested sesame varieties (lines) have good distinction and uniformity, and SSR marker is suitable for variety identification and protection.
国家科技期刊平台
NSTL
万方数据
维普
