Simple Evaluation Method of Tolerance to Salt at Seedling Stage in Soybean
Salt stress seriously affected the production of soybean.Thus screening salt-tolerant soybeans is very important for developing salt resistant cultivars.The objective of this study was to establish a simple method for screening soybean varieties in response to salt stress.The seedlings of four salt-tolerant varieties and four salt-sensitive varieties were grown in vermiculite pouring with 0,100,200,and 250 mmol L-1 NaCl solutions,adding each two days for three times.The SPAD was measured every day.The root,stem and leaf were sampled after eight days of treatment and Na+ content were measured by the atomic absorption spectrophotometer.The results indicated that it is easy to distinguish the level of salt tolerance based on foliar symptoms in both treatment of 200 mmol L-1 and 250 mmol L-1 NaCl.Under 250 mmol L-1 NaCl stress,the Na+ content in leaf for salt-tolerant varieties was lower than that for salt-sensitive varieties.There was a significant correlation between Na+ content and SPAD in salt-sensitive varieties.This experiment established a convenient method of soybean salinity tolerance identification at seedling stage,using the seedlings grown in vermiculite with 200 mmol L-1 and 250 mmol L-1 NaCl treatment for eight days.It will facilitate the screening of germplasm,mechanism study and breeding selection on a large scale.
国家科技期刊平台
NSTL
万方数据
维普
