Development of Genomic SSR Markers in Common Bean and Their Transferability in Cowpea and Adzuki Bean
Transferability analysis of molecular markers has significantly improved their development efficiency and reduced their development cost.A total of 560 novel SSR markers were successfully developed based on common bean genomic sequences,and 421 (75.2％) of those markers generated effective amplification bands from two accessions of the cultivated common bean.Two Vigna species,cowpea and adzuki bean were used to test the transferability and polymorphism of new genomic markers.The resuits indicated that the transferability rate of common bean genomic-SSR in cowpea and adzuki bean was 43.9％ and 38.2％,and the ratio of polymorphism SSR markers in the crops was 34.0％ and 24.8％,respectively.A total of 138 common bean genomic-SSR primers were detected to be highly transferable between two species of Vigna.In addition,the diversity of transferability gene-SSR markers was higher for cowpea and adzuki bean than that of intergenic regions-SSR.These transferable markers are useful for further genetic diversity analysis,genetic linkage map construction,quantitative trait loci annotation,genetic evolution and marker-assisted selection in Vigna species because of their multi-allelic nature,reproducibility,co-dominant inheritance,high abundance in genome.
国家科技期刊平台
NSTL
万方数据
维普
