Kernel size and weight are major determinants of grain yield. For understanding molecular mechanisms of kernel re-lated traits, a recombinant inbred line (RIL) mapping population including 130 families was developed from the cross of two maize elite inbreds, Huangzaosi (HZS) and Mo17. By using the approach of GBS (genotyping-by-sequencing), the high-density genetic map with 1262 bin markers was constructed. QTLs for kernel related traits were identified by stepwise regression (RSTEP-LRT) using Windows QTL ICI-Mapping software under five environments. In total, 30 QTLs were detected under single environment and 11 QTLs were detected under the joint environment. The kernel length major QTL qklen1and the length/width major QTLqklw1 were found in the adjoining regions with a strong genetic effect under three environments. QTLqklen1was located in a region of 210–212 Mb on chromosome 1 with explained 22.60% of phenotypic variance, andqklw1 was located in a region of 207–208 Mb on chromosome 1 with explained 26.79% of phenotypic variance. In addition, for further verification, another introgression population of BC3F1 was developed with Mo17 as the donor parent and HZS as the recurrent parent. The result of the single marker analysis suggested thatqklen1 andqklw1 also had a significantly genetic effect, which is similar to that in the RIL population. The present study provides a good basis for studying genetic mechanism and molecular marker assisted selection for the improvement of kernel related traits in maize.
MaizeKernel related traitQTLHigh-density genetic map
国家科技期刊平台
NSTL
维普
万方数据
