Transcriptome Analysis of Stem Morphogenesis under Shade Stress in Soybean
Maize–soybean relay-cropping system is a key technology in agriculture, studying the molecular mechanisms for shade stress responses of soybean grown in the intercropping system will be useful for soybean improvement by genetic manipulation. An experiment was conducted using two different shade susceptive soybean (Glycine max L.) varieties Nandou 12 and Nan 032-4 to investigate the transcriptome changes in response to shade stress by RNA-seq technology. The results indicated that 287 differ-entially expressed genes in Nandou 12 and 110 genes in Nan 032-4 were significantly affected by shade, and the expression of the genes was mainly up-regulated. Gene ontology analyses showed that differentially expressed genes were enriched in secondary cell wall biogenesis, polysaccharides synthesis, calmodulin binding, hydrolase activity and so on. In the shade treatment, differen-tially expressed genes responded to lignin and auxin biosynthetic processes were up-regulated in Nandou 12; and genes responded to jasmonic acid and ethylene biosynthetic processes were up-regulated, but genes responded to gibberellin metabolic process were down-regulated in Nan 032-4. However, Nandou 12 had more differentially expressed genes than Nan 032-4. The accumu-lateion of polysaccharides in cell wall and acceleration of secondary cell wall formation hindered the radial growth of stem mak-ing stem thinner, meanwhile the increase of hydrolysis enzyme activity accelerated the relaxation of cells, making stem longer. Nandou12 and Nan032-4 also had their particular genes to response shade, but Nandou 12 had more cell wall polysaccharides, lignin content and auxin content to increase the intensity of stem keeping a certain morphological advantage, resulting in higher lodging resistance under the shade, that is a stronger adaptability to shade.
国家科技期刊平台
NSTL
万方数据
维普
