基于AHP-隶属函数法的棉花子叶期耐低钾能力鉴定
Identification of cotton low potassium tolerance based on AHP-membership function method at cotyledonary stage
谭志新 1谢留伟 1李洪戈 2李芳军 1田晓莉 1李召虎1
作者信息
- 1. 中国农业大学农学院作物化控研究中心,北京 100193
- 2. 中国农业科学院棉花研究所品种资源研究室,河南安阳 455000
- 折叠
摘要
挖掘和利用钾高效种质资源、提高钾营养利用效率是解决棉花生产中缺钾问题的重要途经,为建立快速可靠的耐低钾评价体系,应用决策评价模型AHP-隶属函数法在子叶期对 384 份棉花种质材料进行耐低钾评价.结果表明:缺钾条件下(0.03 mmol L-1),子叶缺钾斑相对面积的变异系数最大(96.24%),植株干重变异系数最小(14.73%),子叶与下胚轴夹角、根长、根表面积、根体积、K+浓度、K+积累量的变异系数在 15.72%～31.52%之间.子叶与下胚轴夹角与缺钾斑相对面积显著负相关;根系形态与植株干重和 K+积累量显著正相关.经 AHP-隶属函数法评价,384份棉花种质材料的综合评价值 C 分布于 0.25～0.79 之间,其中无极一枝花(C=0.7868)耐低钾能力最强,巴西 014(C=0.2451)耐低钾能力最弱.系统聚类显示,高度(C值为 0.74～0.79)和中度(C值为 0.33～0.69)耐低钾材料的占比分别为 1.30%和 95.31%,低钾敏感材料(C值为 0.25～0.33)占比 3.39%.种子K+浓度与子叶期耐低钾能力之间的相关性不显著.本研究完善了棉花耐低钾种质资源筛选体系,对 384 份供试种质的子叶期耐低钾能力进行了鉴定,筛选出 5 份耐低钾能力强的种质(无极一枝花、棕 1-61、黑山棉 1 号、秦荔 514、苏联棉 78 系),研究具有一定的理论和应用价值.
Abstract
In order to establish a rapid and reliable evaluation system for low-potassium(K)tolerance in cotton,the combination of decision evaluation model AHP and membership function method was used to evaluate the 384 cotton germplasm resources at the cotyledonary stage.The results showed that,under K deficiency(0.03 mmol L-1),the coefficient of variation of the relative area of K deficiency spots on cotyledons was the largest(96.24%),the coefficient of variation of plant dry weight was the smallest(14.73%).This trait of angle between cotyledons and hypocotyls,root length,root surface area,root volume,K+ concentration,and K+ accumulation ranged from 15.72%to 31.52%.The angle between cotyledons and hypocotyl was significantly negatively correlated with the relative area of K deficiency spots.The characteristics of root morphology were positively correlated with plant dry weight and K+ accumulation.According to the evaluation of AHP-membership function method,the comprehensive evaluation C-value of 384 cotton germplasms varied from 0.25 to 0.79.Among which Wujiyizhihua(C-value = 0.7868)had the strongest resistance to low K,and Brazil 014(C-value = 0.2451)had the lowest resistance to low K.The results of systematic clustering showed that the proportions of highly(C-value＞0.74)and moderately(0.33＜C-value＜0.69)low-K tolerant germplasms were 1.30%and 95.31%,respectively;and the proportion of low-potassium-sensitive(C-value＜0.33)germplasms was 3.39%.The correlation between seed K+ concentration and low K tolerance of cotton at cotyledonary stage was not significant difference.In this study,the screening system of low-potassium tolerant germplasm resources of cotton was improved,the cotyledonary stage potassium tolerance of 384 test germplasms was identified,and 5 germplasms with strong potassium tolerance(Wujiyizhihua,Brown 1-61,Black Mountain Cotton 1,Qinli 514,Soviet Cotton 78)were screened,which provided the theoretical and application value.
关键词
棉花/子叶缺钾斑/根系/干重/钾含量/隶属函数Key words
cotton/potassium deficiency spots on cotyledons/roots/biomass/potassium content/membership function引用本文复制引用
基金项目
国家转基因生物新品种培育重大专项(2016ZX08005-004-008)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据