摘要
甘蓝型油菜是重要的油料作物,而盐胁迫是影响油菜生长发育的主要环境条件之一,可能会造成油菜减产、品质下降甚至死亡.本研究利用半冬性油菜ZS11 作为试验材料,对盐胁迫处理 0、0.25、0.5、1、3、6、12 和 24 h的叶片和根系组织进行转录组测序,通过测得的90份RNA-seq数据,获得了油菜响应盐胁迫的高分辨率时间动态转录表达谱.相关性分析发现,样本在盐胁迫处理 1 h前后具有明显的早期响应与后期响应的聚类差异.利用DESeq2进行差异基因分析,鉴定出根系以及叶片组织响应差异基因分别为 20,462 个和 29,334 个,表明油菜叶片组织的响应程度整体上比根系更剧烈.进一步利用 WGCNA分别构建根系以及叶片组织响应盐胁迫的基因共表达网络,从中筛选出与盐胁迫早期响应阶段显著相关的tan和yellow模块,以及与盐胁迫后期响应阶段显著相关的green和red模块,对其进行GO富集分析,并从中分别筛选出早期以及后期响应盐胁迫的核心转录因子 41 个和 26 个.功能注释显示 4个模块中均存在已知的拟南芥同源基因参与不同阶段的盐胁迫响应,还发现BnWRKY46和BnWRKY57等核心基因在505份盐胁迫处理的油菜群体变异数据中具有丰富的SNPs变异和单倍体类型,表明这些核心转录因子可能是油菜响应盐胁迫的关键候选基因.本研究可为甘蓝型油菜耐盐性改良提供可靠的数据参考和候选基因资源.
Abstract
Brassica napus L.is an important oil crop.Salt stress is one of the major environmental conditions affecting the growth and development of B.napus,which may lead to yield reduction,quality deterioration,and even the death of B.napus.In this study,the B.napus semi-winter cultivar ZS11 was used as the experimental material to perform transcriptome sequencing on the leaf and root tissues with salt stress treatment(0,0.25,0.5,1,3,6,12,and 24 h).The measured 90 RNA-seq data provided the high-resolution time dynamic transcriptional expression spectrum of rapeseed tissues responding to salt stress was obtained.Cor-relation analysis showed that the samples exhibited significant clustering differences in early response and late response before and after 1 h salt stress treatment.Using DESeq2 for differential gene analysis,we identified 20,462 and 29,334 differential genes for the response of root and leaf tissues,respectively,indicating that the overall response of leaf tissue in rapeseed was more se-vere than the root.Furthermore,WGCNA was used to construct gene co-expression networks for the salt stress response in root and leaf tissues,respectively,and tan and yellow modules were significantly related to the early response to salt stress,and green and red modules were significantly related to the late response to salt stress.GO enrichment analysis was then performed,and 41 and 26 core transcription factors responding to salt stress at the early and late stages,respectively,were selected from these net-works.Functional annotation showed that the known Arabidopsis homologous genes involved in salt stress responses at different stages existed in all four modules,and core genes,such as BnWRKY46 and BnWRKY57,had abundant SNPs variation and haplo-types in 505 salt stress-treated rape population variation data,suggesting that these core transcription factors might be key candi-date genes for rape salt stress response.This study provides a reliable data reference and candidate gene resources for improving salt tolerance in B.napus.
基金项目
国家重点研发计划项目(2021YFD160014101-02)
湖北省重点研发计划项目(2020BBB061)
国家科技期刊平台
NSTL
维普
万方数据