The PHR gene is the core transcription factor in the phosphorus signaling regulatory system,responsible for initiating the adaptive response of downstream parts to phosphorus starvation.At the early stage,the transgenic OsPHR2 wheat pure lines with high phosphorus efficiency were obtained,but the molecular mechanism of OsPHR2 improving the phosphorus absorption and utilization efficiency of wheat is still unclear.In order to reveal the molecular mechanism of OsPHR2 improving the phos-phorus uptake and utilization efficiency in wheat,transgenic OsPHR2 wheat pure line with high phosphorus efficiency earlier as the experimental material in this study.Transgenic OsPHR2 wheat and the control were treated with low phosphorus stress when they grew to 4 leaves and 1 heart in hydroponics experiment.Transgenic OsPHR2 wheat and control under low phosphorus stress for 0,6,24,and 72 h were used for transcriptomes analysis by RNA-seq.The differentially expression genes(DEGs)in roots and leaves of transgenic wheat and control were analyzed.There were 22 common DEGs in the roots of transgenic wheat and control under low phosphorus stress for 0,6,24,and 72 h,and there were nine common DEGs in the leaves under four treatments.The functional and pathway enrichments of differentially expressed genes in roots and leaves were also performed by GO and KEGG analysis.The result showed that the number of DEGs in the root of transgenic wheat and control was the highest under low phos-phorus stress for 0 h,followed by 6 h.GO and KEGG enrichment analysis suggested that DEGs were mainly clustered into bio-logical processes such as glucose metabolism,phenylpropanoid biosynthesis,and molecular functions such as nutrient storage activity,ATPase activity,etc.The number of DEGs in the leaves of transgenic wheat and the control was the highest under low phosphorus stress for 72 h.DEGs were mainly clustered into biological processes such as glucose metabolism,organic acid bio-synthesis,as well as molecular functions related to glycosyltransferase activity and cellulose synthase activity.Compared with the control,the key enzyme genes of the defense system such as heme peroxidase and glutathione S-transferase in the root of the transgenic line OsT5-28,as well as the trisphosphate transporter family genes in the leaf were up-regulated before and after low phosphorus stresses.The response of transgenic OsPHR2 wheat to low phosphorus stress was different from that of the control.Transgenic wheat had stronger phosphorus absorption and utilization ability than the control under low phosphorus stress,mainly because OsPHR2 regulated the relative expression level of related genes in wheat.
关键词
低磷胁迫/转基因小麦/转录组/磷素吸收利用效率/差异表达基因
Key words
low phosphorus stress/transgenic wheat/transcriptomics/phosphorus absorption and utilization efficiency/differen-tially expressed genes
维普
万方数据