Genome-wide association study of yield traits and special combining ability in maize hybrid population
马娟 1曹言勇1
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作者信息
1. 河南省农业科学院粮食作物研究所,河南郑州 450002
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摘要
提高产量是玉米育种的长期目标,解析产量相关性状及其配合力的遗传基础对选育高产玉米新品种具有重要意义.本研究选用123份玉米自交系和8份测验种作为亲本,根据NCⅡ(North Carolina design Ⅱ)获得540份杂交种为材料,在新乡和周口试验田调查F1杂交种的单穗粒重、单穗重、百粒重、行粒数等8个产量及构成性状,利用玉米5.5K液相育种芯片检测亲本基因型,推断F1杂交种的基因型,利用BLINK(Bayesian information and link-age-disequilibrium iteratively nested keyway)加性和显性模型开展F1杂交种表型与其特殊配合力(special combining ability,SCA)的全基因组关联分析.结果表明,利用加性和显性模型对F1杂交种分别检测到10个和31个显著关联位点.利用显性模型检测到8个SNPs(single nucleotide polymorphisms)与SCA显著关联.不同性状和模型间共定位位点有7个,其中1个为单穗重与其SCA同时关联位点.通过对主效和共定位SNPs的扫描,共鉴定到26个候选基因,其中转录因子MYBR85、NLP9、PHD3、生长素上调小RNA(SAUR11和SAUR12)、FCS-like锌指蛋白基因FLZ16等可能是控制F1杂交种产量性状与其SCA的重要候选基因.
Abstract
Increasing yield is the long-term goal of maize breeding.It is important to analyze the genetic basis of yield-related traits and combining ability for breeding new maize varieties with high yield.In this study,123 maize inbred lines and eight tester lines were selected as the experimental parents,and 540 hybrids were obtained according to NCⅡ(North Carolina design Ⅱ).The F1 hybrids were investigated at the Xinxiang and Zhoukou experimental fields for eight yield and component traits,including kernel weight per ear,ear weight,100-kernel weight,and kernel number per row.Parental genotypes were detected using maize 5.5K liquid breeding chip,and the genotypes of F1 hybrids were inferred.The additive and dominant models of BLINK(Bayesian information and links-disequilibrium iteratively nested keyway)were used to conduct genome-wide association study of the F1 hybrid phenotypes and special combining ability(SCA).The results showed that 10 and 31 significant association loci were de-tected by additive and dominant models for F1 hybrids,respectively.Eight SNPs(single nucleotide polymorphisms)were signifi-cantly associated with SCA using the dominant model.There were seven co-detected loci among different traits and models,and one of them was co-detected between ear weight and SCA.A total of 26 candidate genes were identified by scanning the ma-jor-effect and co-detected SNPs.Among them,transcription factors MYBR85,NLP9,PHD3,auxin up-regulated small RNA(SAUR11 and SAUR12),and FCS-like zinc finger protein gene FLZ16 may be important candidate genes for controlling yield traits and SCA of F,hybrid.
关键词
玉米/杂交种/特殊配合力/全基因组关联分析
Key words
maize/hybrid/special combining ability/genome-wide association study
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