The bHLH(basic Helix-Loop-Helix)transcription factor plays an important role in plant growth and development.In this study,wheat gene TabHLH112-2B was cloned,which consists of seven exons and six introns,encoding 444 amino acids,and has a typical HLH conserved domain at 315-364 amino acids.The tissue expression pattern analysis showed that TabHLH112-2B was expressed in all tissues at seedling stage,jointing stage,heading stage,and flowering stages.Among them,the relative ex-pression levels in leaves and roots were higher.The cis-acting element analysis showed that the promoter region of TabHLH112-2B contained a variety of cis-acting elements related to plant hormone responses,stress responses,and meristem development.The qRT-PCR exhibited that the relative expression level of TabHLH112-2B was responsive to plant hormones(such as ABA,IAA,MeJA)and abiotic stresses(such as drought,salt,low and high temperatures).Two SNPs were detected in its pro-moter region by genomic sequence polymorphism,which were classified into two haplotypes.A molecular marker was developed based on SNP-682,and association analysis showed that the marker was significantly correlated with the number of spikelet per spike in various environments such as drought and high temperature.Hap-2B-2 was a favorable haplotype with more spikelets per spike.These results of this study provide the valuable genetic resources and technical support for molecular marker-assisted breeding of wheat varieties with high yield and wide adaptability.
关键词
小麦/TabHLH112-2B/分子标记/关联分析/每穗小穗数/优异单倍型
Key words
wheat/TabHLH112-2B/molecular marker/association analysis/the number of spikelet per spike/favorable haplotype
维普
万方数据