首页|大豆巢式关联作图(NAM)群体构建及花色和种皮色遗传分析

大豆巢式关联作图(NAM)群体构建及花色和种皮色遗传分析

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巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用.本研究在前期大豆种质资源评价基础上,利用 35份不同地区来源的代表性种质与中豆 41(公共母本)杂交,构建了一套大豆NAM群体.PCA和聚类分析发现,不同亲本组合的RIL群体基本聚在一起,显示出清晰的遗传结构.利用该NAM群体亲本间花色和种皮色具有显著差异的RIL群体进行全基因组关联分析,定位到 1 个主要位点qFC13-1 与花色显著关联,该位点与W1 位点重合;定位到 12 个位点与种皮色显著相关,其中 9 个位点为 3 种以上方法共定位,3 个位点为 2 种方法共定位,包括 4 个已知位点和 8 个新位点.研究结果表明,构建的NAM群体适于进行大豆相关性状遗传分析,为大豆复杂性状的遗传解析和育种实践提供了良好的基础材料.
Genetic analysis of seed coat and flower color based on a soybean nested asso-ciation mapping population
Nested Association Mapping(NAM)population is widely applied in genetic study and breeding practice in many crops.A NAM panel was constructed by crossing of 35 parental lines with the common maternal lines(Zhongdou 41)based on previous evaluation of soybean germplasm.Principle component analysis and clustering analysis showed that clear genetic structure was observed between subpanel of RIL populations.Genetic analysis was performed on flower color and seed coat color in NAM subpanel with significant difference between paternal and maternal parents,and we found that qFC13-1 was significantly associ-ated with flower color,which coincided with the W1 locus.Twelve loci identified were significantly correlated with seed coat color,among which nine loci were co-located by more than three methods,and the other three loci were co-located by two meth-ods,including four reported loci and eight novel loci.In conclusion,NAM population was suitable for genetic analysis of soybean,which provided material basis for genetic interpretation and breeding practice for complex traits in soybean.

soybeanNAM populationflower colorseed coat colorgenetic analysis

宋健、熊亚俊、陈伊洁、徐瑞新、刘康林、郭庆元、洪慧龙、高华伟、谷勇哲、张丽娟、郭勇、阎哲、刘章雄、关荣霞、李英慧、王晓波、郭兵福、孙如建、闫龙、王好让、姬月梅、常汝镇、王俊、邱丽娟

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长江大学,湖北荆州 434025

农业农村部长江中游作物绿色高效生产重点实验室(部省共建)/长江大学农学院,湖北荆州 434025

中国农业科学院作物科学研究所/农作物基因资源与遗传改良国家重大科学工程/农业农村部北京大豆生物学重点实验室,北京 100081

安徽农业大学农学院,安徽合肥 230036

江西省农业科学院作物研究所,江西南昌 330200

呼伦贝尔市农业科学研究所,内蒙古呼伦贝尔 162650

河北省农林科学院粮油作物研究所,河北石家庄 050035

江苏徐怀地区徐州农业科学研究所,江苏徐州 210031

宁夏农林科学院作物研究所,宁夏银川 750021

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大豆 NAM群体 花色 种皮色 遗传分析

中国农业科学院农业科技创新计划项目(ASTIP)云南省重点科技项目

202202AE090014

2024

作物学报
中国作物学会 中国农业科学院作物科学研究所

作物学报

CSTPCD北大核心
影响因子:1.803
ISSN:0496-3490
年,卷(期):2024.50(3)
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