Isopropyl malate synthase(IPMS)and isopropyl malate dehydrogenase(IPMDH)are important rate-limiting enzymes in leucine biosynthesis.However,their functions in plant growth and development have rarely been reported.In this study,we cloned and performed sequence analysis of GmIPMDH,a homologous gene of Arabidopsis AtIPMDH2 in soybean.GmIPMDH contained a conserved domain of Iso_dh,and the promoter of GmIPMDH contained a large number of light and hormonal respon-sive elements.The qRT-PCR showed that the relative expression level of GmIPMDH in soybean leaves gradually increased with the growth and development of plants.We then performed a function analysis of GmIPMDH by ectopic expression in tobacco and overexpression in soybean.Phenotypic analysis revealed that the overexpression of GmIPMDH significantly promoted flowering of tobacco and soybean.Meanwhile,plant height and nodes number were also increased significantly.Transcriptome analysis displayed that the expression of several flowering-related genes and gibberellin synthesis-related genes were changed in soybean GmIPMDH-overexpression plants.Therefore,we speculated that GmIPMDH may be involved in the gibberellin-mediated flow-ering and plant type architecture regulation.This study elucidates the role of GmIPMDH in the regulation of flowering time and provides a molecular basis for further research on the mechanism of GmIPMDH regulating soybean flowering and plant growth.
维普
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