T.arundinaceum(Tripidium arundinaceum)is an important wild germplasm resource of sugarcane.It is one of the important ways of sugarcane breeding to infiltrate its lineage to improve sugarcane resistance.Karyotyping of the hybrids between sugarcane and T.arundinaceum is beneficial for the efficient utilization of its various superior traits.In this study,we used spe-cies-specific primers for identifying the authenticity of the high generation hybrids between sugarcane and T.arundinaceum,and typed the sugarcane and T.arundinaceum chromosomes by fluorescence in situ hybridization to investigate the inheritance,seg-regation,and structural characteristics of T.arundinaceum in the offspring.These results indicated that 30 clones were true prog-eny with 1-10 T.arundinaceum chromosomes,indicating that their progeny population basically obeyed n+n mode of chromo-some inheritance that accounted for 60%in the entire true progeny population.The probability of recombination at the chromo-somal level between T.arundinaceum and sugarcane was about 16.67%,and the probability of recombination between T.arun-dinaceum and sugarcane of different lineages tended to be the same.Co-localization of S.spontaneum species-specific probes showed that infiltration of T.arundinaceum lineages reduced the proportion of S.officinarum and recombinant chromosomes in modern sugarcane cultivars,and simultaneously increased the proportion of S.spontaneum.In conclusion,analyzing the genetic and structural characteristics of different chromosome sets in the hybrids between sugarcane and T.arundinaceum provides a cytogenetic basis for improving the exploitation of T.arundinaceum germplasm resources in sugarcane breeding.
关键词
甘蔗/斑茅/荧光原位杂交/染色体遗传
Key words
Saccharum/Tripidium arundinaceum/fluorescence in situ hybridization/chromosome inheritance
维普
万方数据