MYB-like transcription factor KAN4(KANADI4)plays an important role in flavonoid synthesis and fiber development in kenaf.In this study,the variety"Fuhong 952"was used as the experimental material to clone and analyze the relative expression pat-tern of HcKAN4 genes,and to investigate the effect of TRV-VIGS-induced silencing of HCKAN4 gene on the expression of key en-zyme genes in flavonoid synthesis pathway in kenaf,the variety'Fuhong 952'was used as the experimental material.Gene cloning showed that the total length of ORF of HcKAN4 gene was 966 bp,encoding 322 amino acids and containing a conserved domain of MYB.Phylogenetic tree revealed that it was closely related to KAN4s of Arabidopsis and Hibiscus.The relative expression pattern indicated that the gene was expressed in different plant tissues,and the transcription level increased with plant growth in kenaf.VIGS-induced gene silencing revealed that the transcription level of 6 HcKAN4 individuals was significantly down-regulated,indi-cating gene silencing successfully.Real-time quantitative PCR demonstrated that the relative level of flavonoid synthesis-related genes,HcCHS,HcF3'5'H,HcANS,and HcANR,were significantly down-regulated,which were 0.51,0.14,0.23,and 0.11 times of those in the control group,respectively,suggesting that the relative expression level of HcKAN4 gene can regulate the biosynthesis of flavonoid in kenaf.These results provide a basis for clarifying the regulation of flavonoid synthesis by MYB transcription factor and give research ideas for improving fiber quality in kenaf.
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