Histone acetylation is an essential type of epigenetic modifications,which is mainly catalyzed by histone acetylases(HATs)and deacetylases(HDACs)and plays a crucial role in plant growth,stress response,and hormone regulation.However,little research information is available about tea plants histone acetylases.We cloned two MCC(MEIOTIC CONTROL OF CROSSOVERS)genes(CsMCC1 and CsMCC2)of HATs family from the'Fuding Dabaicha'tea plant.Meanwhile,the function of these two CsMCC genes was analyzed by bioinformatics methods,qRT-PCR,and subcellular localization.Results showed that the CsMCC1 and CsMCC2 genes were located on chromosomes 1 and 7,encoding alkaline unstable hydrophilic proteins of 257 and 269 amino acids,respectively.The CsMCC genes and protein structure of tea plant were similar to those of the Arabidopsis AtMCC1 gene.The phylogenetic tree and conserved structural domain analysis showed that the MCC protein belonged to the GNAT(GCN5-related N-terminal acetyltransferases)subfamily of HATs proteins,and contained GNAT conserved structures.The evolutionary relationship of CsMCC proteins was closely related to the MCC members in grapes and tomatoes with the highly conserved protein sequences.The subcellular localization in Arabidopsis protoplasts revealed that the CsMCC1 and CsMCC2 proteins were localized on the cytoplasmic membrane.And the promoters of CsMCC1 and CsMCC2 genes contained a number of elements involved in responses to stress,light,and phytohormones.According to transcription data and expression analysis,the relative expression level of CsMCC1 gene was significantly higher in the younger stages of leaf,flower,and root development than the older stages,and CsMCC2 gene was higher in root than other tissues and lasted for a longer time during root development period.The CsMCC1 and CsMCC2 could be regulated by various abiotic stresses(drought,salt,and cold)and exogenous hor-mones(MeJA,GA3,and IAA).In addition,CsMCC proteins could interact with acetyltransferase-related proteins.Hence,CsMCC genes might play roles in tea plant growth and development,and the response to environment through histone acetylation modification.This study explored the basic features and functions of CsMCC,providing the useful theoretical reference for further research on the functions of CsMCC genes in tea plant.
维普
万方数据