The objective of this study is to explore gene differential expression between different sorghum materials under low nitrogen stress conditions and to provide the references for probing into the breeding of low-nitrogen-tolerant sorghum varieties and the molecular mechanism of low-nitrogen-stress tolerance in sorghum.Two low-nitrogen-tolerant sorghum varieties(BSX44 and BTx378)were selected as experimental materials,and both of them were subjected to normal-growth treatment and low-nitrogen-stress treatment respectively before the gene expression of sorghum was detected at seedling stage,heading stage and flowering stage via RNA-Seq technology.The biological functions and metabolic pathways of the differentially expressed genes(DEGs)were analyzed by bioinformatics to screen genes that may be involved in the low-nitrogen regulation,and to under-stand the possible molecular pathways for nitrogen efficient materials in the process of nitrogen absorption and utilization.The results showed that:For BTx378 and BSX44,under normal-growth and low-nitrogen-stress treatments,937 and 787 DEGs were detected at the seedling stage,1305 and 935 at the heading stage,and 1402 and 963 at the flowering stage,for BTx378 and BSX44 respectively.Then the converged DEGs at the three stages were identified,and it was found that 246 genes were differen-tially expressed in the two low-nitrogen-tolerant sorghum varieties at the seedling stage,371 at the heading stage,and 306 at the flowering stage.Furthermore,a total of 28 genes were consistently detected as DEGs at all three stages in the two low-nitrogen tolerant varieties,among which 5 genes were up-regulated and 23 genes were down-regulated.The KEGG analysis of the 28 common DEGs showed that they were mainly enriched in nitrogen metabolism,alanine,aspartic acid and glutamic acid metabo-lism,glycerophospholipid metabolism,and amino acid biosynthesis.This suggested that regulation of the genes in these pathways mainly affects the low nitrogen stress tolerance in sorghum.
维普
万方数据