The study in pot experiments using conventional cultivar Sikang 1(SK1)and hybrid cultivar Sikang 3(SK3)as the experimental materials conducted the changes of Bt insecticidal protein content and nitrogen metabolism related physiological characteristics in Bt cotton fibers at peak boll stage under different low temperature and processing duration.The content of insec-ticidal protein in fibers decreased with the decrease of temperature,and the duration of low temperature treatment significantly affects the content of insecticidal protein compared with the control,the greater decrease of insecticidal protein content was ob-served with the extension of low temperature stress time.The soluble protein content,GOT activity,and GPT activity had a downward trend,while the free amino acid content,peptidase activity,and protease activity had an upward trend.After low tem-perature treatment for 48 hours,there was a significant correlation between those nitrogen metabolism related parameters with insecticidal protein content.Therefore,low temperature stress also decreased protein synthesis,enhanced protein decomposition,resulting in a decrease in soluble protein content,an increase in free amino acid content,and thus a reduced Bt insecticidal protein content,which significantly affected by the duration of low temperature stress.
维普
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