The objective of this study is to investigate the changes in agronomic and physicochemical characteristics of Qingke plants during regeneration and recovery after cutting and to analyze the response patterns of grain and straw yield,as well as quality indica-tors of Qingke.The findings will provide a theoretical basis for the application of the"food-seed-forage trinity"production model of Qingke and the analysis of regulatory mechanisms for plant regeneration and recovery in Qingke.This experiment was conducted from 2021 to 2022,focused on the Qingke varieties Kunlun 18 and Zang 1257.Using normal growth(CK)as the control,a two-factor split-plot design was adopted.Samples were taken at seven developmental stages:0 hour(T0),2 hour(T1),24 hours(T2),72 hours(T3)after cutting,jointing stage(T4),heading stage(T5),and grain filling stage(T6),which aimed to analyze the regeneration rate of stem recovery and changes in antioxidant enzymes in the stubble after cutting and to analyze the yield,quality characteristics of Qingke straw and grain,and the rapid response patterns of endogenous hormones after cutting.The results indicated that cutting in-creased the regrowth of Qingke straw and grain yield.It also leaded to higher levels of crude protein and crude ash content in the stem,while reducing the fiber content.As a result,the relative feeding value of the straw was significantly improved.Cutting promoted the recovery rate of plant height and individual biomass growth in the stem of Qingke.It increased the number of spikes per hectare and 1000-grain weight,resulting in a significant increase in grain yield.Additionally,it leaded to the accumulation of higher levels of crude protein and starch in the grains.After cutting,the levels of superoxide dismutase(SOD)and catalase(CAT)in the stubble of Qingke increased rapidly.It also significantly increased the content of trans-zeatin riboside(TZR)and isopentenyladenosine(iPA)in the stubble.However,it leaded to a significant decrease in the levels of indole-3-acetic acid(IAA)and abscisic acid(ABA)in the stubble.Cytokinins and auxins in the stubble of Qingke may play important regulatory roles in the rapid response of the antioxidant enzyme system and the rapid regeneration process of above-ground parts after cutting.This study provided the foundation for the analysis of the regulatory mechanisms in the post-cutting regeneration and recovery of Qingke plants.
维普
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