Peroxidase(POD)family members play the important roles in regulating plant growth,development,and responding to stress.In order to systematically explore the functions and expression patterns of the TaPOD gene family in wheat(Triticum aes-tivum L.),in this study,we identified the members of the TaPOD gene family in wheat using bioinformatics methods,predicted their physical and chemical properties,promoter homeopathic elements,and evolutionary characteristics,and analyzed their ex-pression patterns in different tissues,exogenous hormones,and stress conditions through wheat transcriptome and RT-qPCR.The results showed that 659 members of the TaPOD gene family were identified from wheat,and their protein sequence lengths were 206-518 amino acids;Phylogenetic analysis showed that the members of wheat TaPOD family were divided into Ⅰ-Ⅶ groups with different numbers of each group.Sequence alignment demonstrated that the members of wheat TaPOD family had five con-servative motifs,with significant differences in gene structure,indicating diversification in functions.Chromosome localization revealed uneven distribution of its quantity on 21 chromosomes of wheat,with the highest number on chromosome 2B.The intraspecific collinearity analysis,showed that there were 396 repetitive events in wheat TaPOD genes,with high homology and very conservative evolutionary process.It was amplified mainly through fragment replication and tandem replication,and the Ka/Ks ratio showed that only four pairs of family members were subjected to positive natural selection pressure.The analysis of cis-regulatory element showed that there were 23 binding elements related to growth,development,and stress resistance in the upstream 2 kb region.Analysis of gene expression patterns showed that most TaPOD genes were highly expressed in wheat roots,and the relative expression level was closely related to hormone induction and abiotic stress.The above results provide a prelimi-nary theoretical basis for further understanding the functions of TaPOD genes in regulating wheat growth,development,and stress.
维普
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