Research on lesion mimic mutant,mining resistance genes,and developing superior disease-resistant new soybean varieties by molecular design breeding methods can contribute to the alleviating the environmental pollution caused by chemical pesticides and drug resistance to disease.In this study,the disease-like rugose leaf mutant NT301 obtained by 60Coγ mutagenesis as the male parent was crossed with W82,KF1,and KF35,respectively,to construct F2 and F2:3 segregating populations.Using SSR and SNP markers,target gene 1(rl1)was narrowed to 937 kb on chromosome 18 with 66 genes and target gene 2(rl2)was narrowed to 130 kb on chromosome 8 with 15 genes.The gene expression patterns of the wild type and NT301 were compared using gene chip technology,and the KEGG pathways of the differentially expressed genes were assessed.Moreover,semi quantitative and quantitative RT-PCR methods were used to analyze the relative expression levels of candidate genes on chromosome 8.The results showed that the relative expression level of Glyma.08G332500 in NT301 was four times higher than the wild type.In contrast,the expression levels of other genes showed no more than double difference.Therefore,we suggest that Glyma.08G332500 may be a candidate gene for NT301.
维普
万方数据