The accuracy estimation of genetic similarity detection of crop varieties is an indispensable supplement and improve-ment to the application of SNP marker method in crop variety detection technology system.In this study,based on the cross-laboratory collaborative validation test data using SNP molecular marker method in 2021,the genetic similarity among wheat varieties and the accuracy of SNP molecular marker method in variety similarity detection were analyzed.The results showed as follows:(1)The overall accuracy of marker locus similarity detection among 55 wheat variety combinations by 10 laboratories was approximately 98%.(2)The genetic relationship between varieties view of GGE biplot delineated the genetic relationship between varieties.The genetic similarity between seven combinations of wheat varieties was over 95%,and the genetic similarity of other combinations was relatively lower.(3)The"trueness-precision"view and"accuracy ranking"view of GGE biplot identified that the similarity detection accuracy of the variety combination Jinmai 47/Linkang 11 was on average,Jimai 22/Yingbo 700 was relatively lower,while Luohan 7/Luohan 11 and other variety combinations were relatively high.(4)Signifi-cant differences were existed in detection accuracy among the 10 laboratories,and the performances in detection trueness,preci-sion and accuracy of two laboratories were significantly worse than those of other laboratories.(5)The tolerance error of the trueness of each laboratory ranged from 1.3%to 1.9%,with an average of 1.5%.The tolerance error of accuracy was distributed between 1.5%and 2.0%,with an average of 1.7%.Among them,the tolerance errors of the detection trueness and accuracy of Lab2 and Lab3 were significantly worse than those of the other laboratories.In this study,the detection accuracy statistical model of SNP marker method in detecting crop variety similarity was constructed to analyze the detection accuracy and the correspond-ing tolerance error of variety combination in different laboratories,and the GGE biplot techniques were adopted to visualize the detection trueness,precision,and accuracy,so as to verify the accuracy and reliability of the detection method for variety locus similarity in each laboratory.Therefore,the findings in this study could provide the theoretical support and application examples for the accuracy evaluation of SNP marker detection technique system for genetic similarity among crop varieties.
维普
万方数据