摘要
生长调控因子(growth-regulating factor,GRF)在植物的生长发育、逆境响应和激素信号转导中起着重要的调控作用.系统分析小麦及其祖先物种GRF转录因子家族成员在基因组上的分布、结构、进化以及表达特性,对于深入研究GRF家族的生物学功能和小麦的进化具有重要的意义.本研究利用生物信息学方法,对乌拉尔图小麦、拟斯卑尔脱山羊草、粗山羊草、栽培二粒小麦和普通小麦 5 个物种的GRF成员进行全基因组鉴定,并对其理化性质、系统发育关系、基因结构、启动子顺式作用元件以及表达特性进行了分析.结果表明,乌拉尔图小麦、拟斯卑尔脱山羊草、粗山羊草、栽培二粒小麦和普通小麦中分别有 15、12、19、29 和 53 个GRF成员,通过种间共线性分析发现,TtGRFs分别有 18 个和 29 个成员与TuGRFs和AesGRFs具有共线性,TaGRFs分别有 36 个和 37 个成员与TtGRFs和AetGRFs具有共线性.启动子顺式作用元件预测发现GRF基因具有基本的转录元件以及一些与生长发育和逆境响应的结合元件.RT-qPCR 分析表明,多数 GRF 基因在外源 IAA、GA 和干旱胁迫条件下呈上调表达趋势,而在高温胁迫条件下呈下调表达趋势,表明 GRF 家族成员在响应激素和逆境胁迫中有重要作用.系统进化分析表明,小麦与其祖先物种之间的 GRF 成员存在保守且复杂的进化关系.上述结果为 GRF 转录因子家族的进化及其功能研究提供了理论基础.
Abstract
Growth-regulating factors(GRFs)play important roles in plant growth,stress response,and hormone signal transduc-tion.Systematic analysis of the distribution,structure,evolution,and expression characteristics of the GRF transcription factor family members in the genome of wheat and its ancestral species is of great significance for in-depth research on the biological functions of GRF family and the evolution of wheat.In this study,bioinformatics methods were used to identify the whole ge-nome of GRF members from five species(Triticum urartu,Aegilops speltoides,Aegilops tauschii,Triticum turgidum,and Triti-cum aestivum),and their physical and chemical properties,phylogenetic relationships,gene structure,promoter cis-regulatory element,and expression characteristics were also analyzed.The results showed that there were 15,12,19,29,and 53 GRF mem-bers in Triticum urartu,Aegilops speltoides,Aegilops tauschii,Triticum turgidum,and Triticum aestivum,respectively.Through interspecific colinearity analysis,we found that 18 and 29 members of TtGRFs were colinear with TuGRFs and AesGRFs,and 36 and 37 members of TaGRFs were colinear with TtGRFs and AetGRFs,respectively.The prediction of promoter cis-regulatory element found that GRF gene had basic transcription elements and some binding elements with growth,development,and stress response.RT-qPCR analysis revealed that most GRF genes up-regulated under exogenous IAA,GA,and drought stress,but down-regulated under high temperature stress,indicating that members of the GRF family exerted a crucial influence in hormone response and stress.Phylogenetic analysis evidenced that there was a conserved and complex evolutionary relationship between the GRF members of wheat and its ancestral species.The above results provide a theoretical basis for the evolution and functional research of the GRF transcription factor family.
基金项目
国家自然科学基金项目(32372227)
河南省自然科学基金项目(222300420430)
河南省高等学校青年骨干教师培养计划(2021GGJS050)
维普
万方数据