Gibberellin 3-beta-dioxygenase(GA3ox)is one of the key enzymes involved in gibberellin biosynthesis,which can regulate plant growth and development by affecting gibberellin formation.There were no systematic studies in peanut genome.In this study,AhGA3ox family genes were identified from the genome database of cultivated peanut species by bioinformatics,and the distribution,structure,evolutionary characteristics,physical and chemical properties,promoter cis-acting elements were also analyzed.The relative expression pattern of AhGA3oxs in different peanut tissues was analyzed by qRT-PCR,and the relative expression level of in shell of two peanut lines with different pod size were also analyzed.The results showed that 7 AhGA3oxs were distributed on 7 chromosomes of peanut,all of which were composed of 1 intron and 2 exons.All the AhGA3ox proteins contained 1 DIOX_N domain and 1 2OG-FeII_Oxy domain.Phylogenetic analysis showed that they were closely related to GA3ox proteins of soybean,.The AhGA3oxs showed obvious tissue expression specificity in root,stem,leaf,flower and peg.The expression levels of AhGA3oxs were not only different in different development stages of the peanut shell,but also different at the same development stage of the two lines.Interestingly,the relative expression levels in large pod lines were significantly higher than those in small pod lines at most development stages.Therefore,we predict that the gene expression of AhGA3ox gene family may promote the formation of large pod.
维普
万方数据