In order to explore the mechanism of exogenous salicylic acid in improving the resistance of wheat stripe rust,using,the untreated wheat seedlings(control),stripe rust wheat seedlings(SR),and salicylic acid treated stripe rust wheat seedlings(SA-SR)as the experimental materials were investigated using Zhoumai 18.After 15 days of treatment,the wheat seedlings were identified for disease resistance,amino acid detection,transcriptome sequencing,and fluorescence quantification.The results indicated that the exogenous salicylic acid could significantly reduce the pathogenicity of stripe rust in wheat seedlings.The content of amino acids in leaves in the SR group was generally reduced,while there was no significant difference between the SA-SR group and the control group pretreated with salicylic acid,indicating that the content of amino acids in leaves was significantly reduced due to wheat stripe rust.GO enrichment indicated that the differentially expressed genes of SR and SA-SR groups were enriched in biological processes related to photosynthesis involving cell components such as chloroplasts and thylakoids.KEGG pathway enrichment showed that both SR and SA-SR subgroups were significantly enriched in the MAPK signaling pathway and the biosynthesis of secondary metabolites played an important role in plant disease resistance or stress resistance.
维普
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