玉米多叶矮化突变体lyd1的鉴定与基因克隆
Identification and gene cloning of leafy dwarf mutant lyd1 in maize
苏帅 1刘孝伟 1牛群凯 1时子文 1侯雨微 1冯开洁 1荣廷昭 1曹墨菊1
作者信息
- 1. 四川农业大学玉米研究所 / 农业部西南玉米生物学与遗传育种重点实验室, 四川成都 611130
- 折叠
摘要
玉米株高降低通常是由节间数目减少、节间长度变短或二者共同作用所致.而本研究在基因编辑后代中发现的玉米多叶矮化突变体 lyd1 却表现为节间数目显著增加,株高显著降低.lyd1 株高仅为 93.10 cm,与野生型KN5585 的株高 159.95 cm相比,降低了 41.79%,差异达到极显著水平.然而lyd1 叶片数平均达到 27.8 片,相较野生型平均 17.8 片叶,增加 56.18%,差异达到极显著水平.遗传分析表明,lyd1 的突变表型由 1 对隐性核基因控制,通过图位克隆将控制多叶矮化性状的基因定位于玉米 3 号染色体标记Indel10 和Indel11 之间,物理距离 0.74 Mb.对定位区间内 13 个基因(不包含假基因)的序列进行测序,发现仅ZmTE1 在第 4 外显子出现 1 个A碱基的替换,其他基因无差异.ZmTE1 编码一个RNA结合蛋白,氨基酸的替换发生在第 3 个RNA结合结构域内(RRM3),导致天冬氨酸转变为缬氨酸.突变体lyd1 的突变位点与已报道的te1-mum1、te1-mum2、te1-mum3、zm66 不同,lyd1 的发现为进一步解析玉米叶片和节间发育平衡的遗传机制提供了宝贵的材料.
Abstract
The decrease of plant height in maize is usually caused by the decrease in the number of internodes,the shortening of internodes or the combination of both.However,in this study,the mutant leafy dwarf1(lyd1)found in the progeny of gene editing,exhibited more leaves and shorter stature.Quantitative measurements indicated the plant height of mutant lyd1 was only 93.10 cm,the plant height of wild-type KN5585 was 159.95 cm.The plant height was significantly reduced by 41.79%in mutant lyd1 com-pared with the wild type KN5585.The wild type KN5585 produced an average of 17.8 leaves at maturity stage,whereas mutants lyd1 produced 27.8 leaves.The number of leaves were significantly increased by 56.18%in mutant lyd1 compared with the wild type.Genetic analysis showed that the mutation phenotype of lyd1 was controlled by a pair of recessive nuclear genes.We applied a map-based cloning strategy to identify the gene responsible for the lyd1 phenotype.The gene was located between Indel10 and Indel11 on maize chromosome 3,and the physical distance was 0.74 Mb.Gene sequencing analysis of 13 genes(excluding pseu-dogenes)within the interval revealed that one base A was substituted in the fourth exon of ZmTE1,and there was no significant difference in other genes.ZmTE1 encoded an RNA-binding protein.The amino acid substitution was in the third RNA binding domain(RRM3),resulting in the conversion of aspartic acid to valine.The mutation sites of the mutant lyd1 were different from te1-mum1,te1-mum2,te1-mum3,and zm66 in previously reported.The discovery of lyd1 provides valuable materials for further analysis of the genetic mechanism of the balance between leaves and internodes development in maize.
关键词
玉米/叶片数量/节间长度/基因定位Key words
maize/the number of leaves/the length of internodes/gene mapping引用本文复制引用
基金项目
四川省科技计划(2021YFYZ0011)
四川省科技计划(2021YFYZ0017)
四川省科技计划(MZGC20230108)
四川农业大学学科建设专项研究支持计划()
出版年
2024
国家科技期刊平台
NSTL
万方数据