耐亚磷酸盐除草剂转基因油菜的创建和抗性评价
Developing and resistance assessing of phosphite-tolerant herbicide transgenic Brassica napus L.
钟元 1朱天宇 1戴成 1马朝芝1
作者信息
- 1. 华中农业大学作物遗传改良全国重点实验室 / 国家油菜工程技术研究中心 / 洪山实验室, 湖北武汉 430070
- 折叠
摘要
在油菜的生产过程中,大量伴生的杂草严重影响了其产量和品质.由于近年来田间抗除草剂杂草数量的增加,除草剂的选择正在迅速减少,这影响了未来农业的可持续发展.植物可以通过正磷酸盐(Pi)转运蛋白吸收亚磷酸盐(Phi),但Phi不能被代谢为作物的磷肥料,导致植物生长受到抑制.此前,从罗尔斯通菌属(Raltsonia sp.strain 4506)中分离出了ptxD(phosphite dehydrogenase)基因,其 139 位的酪氨酸突变为谷氨酰胺(Y139Q)的突变蛋白ptxDQ催化Phi转化为Pi的活性显著提高.为了评价ptxDQ/Phi系统对甘蓝型油菜杂草的防治效果,本研究获得了带有密码子优化的ptxD(Y139Q,ptxDQ)基因的转基因甘蓝型油菜.在以Phi为单一磷元素的条件下,ptxDQ转基因油菜可以正常生长,体内 Pi 含量显著提高,且 Pi饥饿响应基因(PT21 和 PT24 等)的表达水平被显著抑制,说明 ptxDQ在油菜体内可以促使 Phi转化为 Pi.此外,ptxDQ转基因油菜具有比野生型油菜和单子叶杂草(狗尾草)更强的生长竞争优势.综上所述,ptxDQ/Phi是一种有效的油菜磷利用和杂草控制系统,既可以抑制杂草的生长,又可以为作物提供充足的 Pi营养,从而提高了农业的可持续性.
Abstract
During the production of Brassica napus,the yield and quality of the crop are seriously affected by a large number of accompanying weeds.Herbicide control options are rapidly diminishing due to the recent increase in the number of herbi-cide-resistant weeds in fields,which affects the sustainable development of agriculture in the future.Plants can take up phosphite(Phi)via orthophosphate(Pi)transporters,but the Phi cannot be metabolized and used as a phosphorus fertilizer for crops,result-ing in plant growth inhibition.Previously,a ptxD gene isolated from Ralstonia sp.4506,and the mutant protein ptxDQ at position 139,which had tyrosine mutation to glutamine(Y139Q),significantly improved the conversion activity of Phi to Pi.To evaluate the efficacy of ptxDQ/Phi-based weed control system in Brassica napus,we generated transgenic B.napus plants with a codon-optimized ptxD(Y139Q,ptxDQ)gene.Ectopic expression of ptxDQ confered the ability to convert Phi to Pi,resulting in improving plant growth in the presence of Phi.Pi-starvation-induced genes(e.g.PT21 and PT24)were suppressed in the ptxDQ transgenic lines by supplying Phi,indicating that ptxDQ can transform Phi into Pi in rapeseed.In addition,ptxDQ transgenic Bras-sica napus had a greater competitive growth advantage over wild-type Brassica napus and monocotyledonous weeds(Setaria glauca).In conclusion,the ptxDQ/Phi system provided an effective alternative for suppressing the weed growth while providing adequate Pi nutrition to the crops,which in turn improved the sustainability of agriculture.
关键词
甘蓝型油菜/除草剂/亚磷酸盐/ptxDQKey words
Brassica napus/herbicide/phosphite/ptxDQ引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据