陆地棉重组自交系再生能力和遗传转化效率筛选
Screening of regeneration capacity and genetic transformation efficiency in recombinant inbred lines of Gossypium hirsutum L.
乐愉 1王涛 1张献龙 1林忠旭1
作者信息
- 1. 华中农业大学植物科学技术学院 / 作物遗传改良全国重点实验室, 湖北武汉 430070
- 折叠
摘要
转基因工程育种是棉花种质创新的有效手段,为了拓展陆地棉可再生基因型,丰富棉花转基因受体,本研究以湖北省高产阔叶棉品种'鄂棉 22'(E22)为母本、高再生能力的鸡脚叶品种'豫早 1 号'(YZ1)为父本,通过单籽传法构建了F9 重组自交系群体(YE);利用棉花中比较成熟的IBA+KT(IK)和 2,4-D+KT(DK)2 种不同激素组合的培养体系,分别对重组自交系群体的 164 个家系的愈伤组织诱导率(CIF)、愈伤组织继代繁殖力(CSC)、愈伤组织的出胚率(CRE)以及愈伤组织出胚时间(CET)进行比较,共获得12个可再生的阔叶棉家系;利用目前成熟的棉花遗传转化体系对可再生阔叶棉家系进行遗传转化效率分析,同时对其进行田间农艺性状考察,最终获得了一个遗传转化效率为82.9%并且农艺性状更优良的家系YE3.本研究为棉花的遗传转化及基因功能研究拓展了陆地棉基因型种质资源.
Abstract
Transgenic engineered breeding is an effective way of cotton germplasm innovations.In order to expand the renewable genotypes of upland cotton and to enrich cotton transgenic receptors,we constructed an F9 recombinant inbred population(YE)by the single-seed descent method using the high-yielding broad leaf cotton variety Emian 22(E22)from Hubei province as the male parent and the high regeneration-capable okra leaf variety Yuzao 1(YZ1)as the female parent.We compared the callus induction rate(CIF),callus secondary fecundity(CSC),embryo rate of callus(CRE),and callus embryo time(CET)of 164 recombinant inbred lines using two different hormone combinations of IBA+KT(IK)and 2,4-D+KT(DK),respectively,which were mature tissue culture systems in cotton.A total of 12 regenerable broad leaf lines were obtained and evaluated for the genetic transforma-tion efficiency using the current mature genetic transformation system in cotton;meanwhile,agronomic traits of these lines were investigated.Finally,a family line YE3,with a genetic transformation efficiency of 82.9%and better agronomic traits,was ob-tained.This study develops a new genotype of upland cotton,which will facilitate the genetic transformation and gene function research of cotton.
关键词
棉花/重组自交系/体细胞胚胎发生/再生能力/遗传转化效率Key words
cotton/RILs/somatic embryogenesis/regeneration capacity/transformation efficiency引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据