摘要
维生素E(Ve)是大豆油中一种天然抗氧化剂,是评价大豆油营养价值的重要指标.本研究利用含有 264 份的大豆自然群体在2021年和2022年测定了籽粒中α-、γ-和δ-生育酚含量,并进行全基因组关联分析(Genome-wide association study,GWAS).本研究共检测到 199 个与大豆Ve含量显著关联的SNP位点,其中 9 个可在 2 个环境或者 2 个性状被重复检测到,分别位于 3 号、7 号、11 号、12 号、13 号、15 号、17 号和 18 号染色体上.其中位于 7 号染色体上的显著关联信号是控制α-生育酚含量的主效位点,可在 2 年环境中被检测到,表型变异解释率为 9.83%.对该位点候选基因进行筛选,获得一个编码myb转录因子的基因Glyma.07G054000,可能是这个位点的效应基因.另外,在 12 号染色体上得到 2 个编码γ-生育酚甲基转移酶的基因Glyma.12G014200 和Glyma.12G014300,有可能是影响Ve含量的重要基因.本研究结果有助于解析大豆籽粒Ve含量的遗传基础及其调控机制,为大豆品质遗传改良奠定了基础.
Abstract
Vitamin E(Ve)is a natural antioxidant in soybean oil and an important index to evaluate the nutritional value of soy-bean oil.In this study,α-,γ-,and δ-contents of seed were determined from a natural soybean population containing 264 germ-plasm resources in 2021 and 2022,and genome-wide association study(GWAS)was conducted.A total of 199 SNPs significantly associated with soybean Ve content were detected in this study,among which 9 SNPs that could be repeatedly detected in two different environments or two traits,which located on chromosomes 3,7,11,12,13,15,17,and 18,respectively.Among them,the significant association signal on chromosome 7 was a major effect site controlling α-tocopherol content,which can be detected in two environments,with the explanation rate of phenotypic variation 9.83%.By screening the candidate genes of this site,Glyma.07G054000 encoding myb transcription factor was obtained,which may be the effect gene of this site.In addition,two genes encoding γ-tocopherol methyltransferase Glyma.12G014200 and Glyma.12G014300 obtained on chromosome 12,may be important genes affecting Ve content.The results of this study are helpful to analyze the genetic basis and regulatory mechanism of Ve content in soybean seed,and lay a foundation for genetic improvement of soybean quality.
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