Potato cultivars are not tolerant to low temperatures and frost,which directly affects the growth and development of plants and tubers,thus affecting potato yield.Wild potato species,such as S.boliviense,have abundant resources for cold frost resistance,which are important resources for improving cultivated potato varieties.Based on cold resistance identification of dif-ferent SS.boliviense strains,we screened and obtained the excellent strain BLV29-2(S.boliviense)with comprehensive traits.It was then crossed and backcrossed with the diploid cultivar ED25,resulting in interspecific hybrids with stronger cold resistance.Inter-specific hybrid strains that had shown significant improvement in cold tolerance were treated with recycled colchicine.Cold tolerance and agronomic traits were assessed in some of the strains,and the results showed that all the doubled materials had sig-nificant increases in plant height,pollen grain diameter,and individual potato weight.Most of the materials showed there was no significant change in cold tolerance before and after doubling,but significantly improved compared to the cultivated species con-trol.By crossing the treated strain T-FT073-4-7 with the superior tetraploid cultivar Huashu 13,we observed segregation of cold resistance in the offspring,with the 47％of the materials showing a preference towards the maternal parent T-FT073-4-7 and significantly higher cold resistance compared to the paternal cultivar.Through further field evaluations of agronomic traits,we identified selected breeding materials with the improved overall traits and the significantly enhanced cold resistance.This study successfully introduced the excellent cold resistance of the diploid wild species SS.boliviense into the tetraploid cultivar,thus ad-dressing the deficiency of the existing cultivar in terms of low-temperature sensitivity.These findings provide an important foun-dation for the selection and further improvement of cold-resistant genetic breeding materials.
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