马铃薯ARM基因家族的全基因组鉴定及表达分析

扫码查看

原文链接

万方数据
维普

中文摘要：ARM蛋白重复序列(Armadillo repeats)广泛存在于高等植物中,它们参与多种细胞过程,如信号转导、核转运以及对多种生物/非生物胁迫的响应.本研究在马铃薯(Solanum tuberosum L.)全基因组水平下鉴定出了 54个马铃薯ARM基因家族成员(StARMs),它们不均匀的分布在12条染色体上.根据其蛋白结构和系统发育特征,将54个StARMs分为3个亚家族.片段重复事件在马铃薯ARM基因家族的扩展中起主要作用.共线性分析发现,StARMs与番茄(Solanum lycopersicum)、拟南芥(Arabidopsis)、甘蓝(Brassica oleracea)、水稻(Oryza sativa)、玉米(Zea mays)分别有51对、17对、25对、6对和10对直系同源基因,这些基因均在纯化选择下进化.RNA-seq数据分析发现,4个StARM基因在匍匐茎中特异表达,2个StARM基因在根和心皮中特异表达,1个StARM基因在块茎中特异表,还有一些StARM基因参与了马铃薯对生物/非生物胁迫的响应.此外,本研究对3个不同颜色马铃薯块茎组织(薯皮和薯肉)进行了 RNA-seq测序,分析了 54个StARMs在不同颜色马铃薯块茎组织中的表达模式,并利用qPCR分析了 StARMs在3个不同颜色块茎杂交子代薯肉中的相对表达量,筛选出了 4个可能参与马铃薯块茎花色素苷生物合成的候选基因.本研究为进一步了解StARM基因家族的特征,深入分析StARM基因在马铃薯抵御生物/非生物胁迫和调控块茎花色素苷生物合成中的功能提供了理论依据.

外文标题：Genome-wide identification and expression analysis of ARM gene family in potato(Solanum tuberosum L.)

外文摘要：The armadillo repeats(Armadillo repeats)are widely distributed in higher plants and are involved in a variety of cellu-lar processes such as signal transduction,nuclear transport,and the response to various biotic/abiotic stresses.In this study,54 potato ARM gene family members(StARMs)were identified at the genome-wide level of potato(Solanum tuberosum L.),and they were unevenly distributed on 12 chromosomes.Based on their protein structure and phylogenetic characteristics,54 StARMs were divided into three subfamilies.Segmental duplication events play a major role in the expansion of potato StARM gene fam-ily.Collinearity analysis showed that there were 51,17,25,6,and 10 orthologous gene pairs between StARMs and tomato,Arabi-dopsis,cabbage,rice,and maize,respectively,which evolved under purification selection.RNA-seq data analysis showed that four StARMs genes were specifically expressed in the stolon,two StARMs were specifically expressed in the root and carpel,and one StARM gene was specifically expressed in the tuber.Some StARM genes were involved in potato response to biotic/abiotic stresses.In addition,we performed RNA-seq on three different colored potato tuber tissues(skin and flesh)and analyzed the rela-tive expression pattern of 54 StARMs genes in different colored potato tuber tissues and the StARMs in potato flesh in three differ-ent colored potatoes by qPCR.Four candidate genes that may be involved in anthocyanin biosynthesis in potato tubers were screened.This study provides a theoretical basis for further understanding the characteristics of the StARM gene family and fur-ther analyzing the function of the StARM gene in potato resistance to biotic/abiotic stresses and regulation of anthocyanin biosyn-thesis in tubers.

外文关键词：

potatoARM gene familybiotic/abiotic stressanthocyanin biosynthesisthe relative expression pattern

作者：

刘震、陈丽敏、李志涛、朱金勇、王玮璐、齐喆颖、姚攀锋、毕真真、孙超、白江平、刘玉汇

展开 >

作者单位：

甘肃农业大学省部共建干旱生境作物学国家重点实验室,甘肃兰州 730070

甘肃农业大学农学院,甘肃兰州 730070

甘肃省教育厅,甘肃兰州 730030

关键词：

马铃薯 ARM基因家族生物/非生物胁迫花色素苷生物合成表达分析

基金：

甘肃省科技计划甘肃省科技计划现代农业产业技术体系建设项目甘肃省科技重大专项中央引导地方科技发展资金项目省部共建干旱生境作物学国家重点实验室开放基金甘肃农业大学"伏羲人才"计划甘肃农业大学公招博士科研启动基金

项目编号：

22JR5RA87022JR5RA834CARS-09-P1422ZD6NA00923ZYQJ304GSCS-2021-Z02Gaufx-02Y04GAU-KYQD-2020-11

出版年：

2024

DOI：

10.3724/SP.J.1006.2024.34121

作物学报

中国作物学会　中国农业科学院作物科学研究所

作物学报

CSTPCD北大核心

影响因子：1.803

ISSN：0496-3490

年,卷(期)：2024.50(6)