The IGT gene family is involved in the regulation of crop plant type,and LAZY belongs to the IGT subfamily.By comparing the amino acid sequences of six Arabidopsis LAZY members as'seeds'in the cassava genome,a total of 8 LAZY genes were identified in cassava,among which MeLAZY1c is highly homologous to AtLAZY1,which regulates branching an-gles.Based on this,MeLAZY1c had the highest transcription level in the stem by qRT-PCR andpMeLAZY1c was deeply stained in the vascular bundle by GUS staining,using MeLAZY1c as the experimental materials in this study.Eight photorespon-sive/regulatory elements were found in the MeLAZY1c promoter,and we subsequently found that darkness significantly inhib-ited the relative expression level of MeLAZY1c.At the same time,gene editing of MeLAZY1c was performed and 19 homozygous edited lineages.The phenotype of MeLAZY1c mutants was observed after seedling transplantation,compared to the SC8 wild-type,the main stems of MeLAZY1c mutants had crawing growth,and the stem skin cells at the curved part were distorted and deformed with different sizes.The number of cells at 1 mm near the ground was about 1.5 times that of cells at the far ground.In conclusion,these results indicates that MeLAZY1c plays an important role in the establishment of upright/creeping growth of cassava.
关键词
木薯/株型调控/LAZY/光响应/匍匐/基因编辑
Key words
cassava/plant type regulation/LAZY/light response/crawling/gene editing
维普
万方数据