In order to understand the function of cyclophilin family OsCYP22 from Oryza sativa L.,the yeast two hybrid bait vector of OsCYP22 was constructed and proteins interacting with OsCYP22 were screened by yeast two hybrid.The results showed that noself-activating activity and toxicity of OsCYP22 bait vector to yeast cells were detected.,among which 38 posi-tive colonies were obtained by yeast two hybrid screening.Combining sequencing analysis and bioinformatics methods,20 candidates that may interact with OsCYP22 were screened.Further validation of the full-length interactions between OsCYP22 and OsCSN5 and OsRUB1 related to the regulation of plant root growth was conducted.The results showed that OsCSN5 in-teracted with OsCYP22 in yeast.This study provides a theoretical basis for further study of the biological function of OsCYP22.
维普
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