摘要
本研究利用小麦 55K SNP(55K single-nucleotide polymorphism)芯片和DArT(diversity array technology)标记对Avocet/Chilero和Avocet/Huites构建的两个F6 重组自交系群体(recombinant inbred line,RIL)进行了小麦籽粒蛋白质含量(grain protein content,GPC)、湿面筋含量(wet gluten content,WGC)和沉降值(sedimentation value,SV)的QTL(quantitative trait loci)定位.共鉴定到 68 个与小麦籽粒蛋白质含量、湿面筋含量和沉降值相关的QTL,表型贡献率为3.60%～22.53%,其中,位于 3A(2)、4D、5D(2)、6A(8)和 7B染色体上的 14 个QTL可在多环境下被重复检测到.此外,在 3A、3D、4B、5D、6A(2)和 7B染色体上检测到 7 个QTL簇,位于 3AS染色体 9.32～60.01 Mb和 6AS染色体38.47～82.95 Mb的稳定QTL簇C3A和C6A.2,同时与小麦籽粒蛋白质含量、湿面筋含量和沉降值显著相关,分别解释了 6.55%～14.21%和 3.83%～22.53%的表型变异.同时,在 2 个QTL簇中筛选到 16 个可能与籽粒蛋白质含量相关的候选基因,并根据候选基因开发了可供育种利用的 KASP标记 CGPC-6A-KASP-1 和 CGPC-6A-KASP-2.本研究为小麦籽粒品质相关性状的遗传改良提供了新的QTL位点和KASP标记,为分子标记辅助育种提供依据与参考.
Abstract
We utilized a 55K single-nucleotide polymorphism(55K SNP)array and diversity array technology(DArT)to identify QTLs for grain protein content(GPC),wet gluten content(WGC),and sedimentation value(SV)in two F6 recombinant inbred lines derived from Avocet/Chilero and Avocet/Huites.Sixty-eight QTLs were identified related to grain protein content,wet gluten content,and settlement value,explaining 3.60%-22.53%of the phenotypic variances.Fourteen QTLs were found to be present in multiple environments,located on chromosomes 3A(2),4D,5D(2),6A(8),and 7B,respectively.Additionally,seven QTLs clusters were detected on chromosomes 3A,3D,4B,5D,6A(2),and 7B,respectively.Two stable QTL clusters,C3A and C6A.2,identified in the physical intervals of 9.32-60.01 Mb and 38.47-82.95 Mb,respectively,were significantly associated with grain protein content and wet gluten content.These clusters accounted for 6.55%-14.21%and 3.83%-22.53%of the phenotypic variances for grain protein content,wet gluten content,and sedimentation value.Meanwhile,a total of 16 candidate genes associated with grain protein content were predicted within the two stable QTL clusters.Moreover,two KASP marker,CGPC-6A-KASP-1 and CGPC-6A-KASP-2,were developed based on the candidate genes.The findings of this study provide support for the identification of new QTL and KASP markers that can contribute to the genetic improvement of grain quality-related traits in wheat.These re-sults also offer valuable insights for marker-assisted breeding in wheat.
基金项目
河南省科技重大专项(231100110300)
神农种业实验室"一流课题"项目(SN01-2022-01)
维普
万方数据