摘要
室内生物测定是农作物除草剂耐受性鉴定的常用方法,已广泛应用于大豆、棉花等作物对草甘膦、氯嘧磺隆等除草剂的耐受性研究,但莠去津室内生测方法及大豆对莠去津耐受性相关研究鲜见报道.本研究以莠去津对大豆萌发期各单项指标为评价指标,分析莠去津胁迫条件下大豆发芽势、发芽率、发芽指数、活力指数、根长等性状的变化,建立大豆莠去津耐受性室内生测方法,确定 3.5 mL L-1 是大豆萌发期莠去津耐受性筛选的适宜浓度;对 159份大豆种质进行萌发期莠去津耐受性鉴定表明,各指标相对值的变异系数为发芽相对莠去津胁迫率>相对芽长>相对发芽指数>相对活力指数>相对发芽势>相对发芽率;根据T值的聚类分析,将供试种质分为 4 个莠去津耐性级别,共鉴定出高耐种质 9 份、中耐种质 50 份、低耐种质 70 份和敏感种质 30 份;黄豆、花色豆、垦丰 13、南农 99-6 等 9个种质为莠去津耐受性综合能力优异的种质资源.采用多元逐步回归分析法,建立大豆萌发期耐莠去津评价模型Y=-0.155+0.004X2+0.001X3+0.001X4+0.002X5(P=0.000).本研究为培育耐莠去津育种的亲本选配、后代选择及大豆耐莠去津相关基因挖掘提供了理论依据、材料和技术支撑.
Abstract
Laboratory bioassay is a common method to identify crop herbicide tolerance,which was widely used in soybean,cot-ton,and other crops to study the tolerance of glyphosate,chlorsulfuron and other herbicides,but there are few reports on labora-tory bioassay method and soybean tolerance to atrazine.In this study,the effects of atrazine on the germination stage of soybean were evaluated,and the changes of germination potential,germination rate,germination index,vitality index,and root length of soybean under atrazine stress were analyzed.A laboratory bioassay method was established for the atrazine tolerance of soybean,and 3.5 mL L-1 was determined as the appropriate concentration for screening atrazine tolerance of soybean at germination stage.The tolerance of atrazine to 159 soybean germplasms at germination stage was identified.The results showed that the relative coefficient of variation of each index was the relative atrazine stress rate,the relative bud length,the relative germination index,the relative vitality index,the relative germination potential,and the relative germination rate.According to the T-value cluster analysis,the tested germplasm was divided into 4 atrazine tolerance grades,and 9 high-tolerant,50 medium-tolerant,70 low-tolerant,and 30 sensitive germplasm were identified.Nine germplasm,including soybean,fancy bean,Kenfeng 13,and Nannong 99-6,had excellent tolerance to atrazine.This study provides the theoretical basis,material and technical support for the selection of parents,the selection of progeny and the mining of genes related to atrazine tolerance in soybean.
基金项目
海南省重点研发计划(ZDYF2021XDNY275)
维普
万方数据