β-glucan is the main functional component of oats for health care,and improving its content is of great significance to the production of high-quality oats.In this study,in order to promote the effective utilization of high β-glucan oat germplasm re-sources and the discovery of related genes,a genetic linkage map containing 21 linkage groups and 5032 bin markers was con-structed by using resequencing technology with the 223 RIL8 populations derived from the high β-glucan variety Xiayoumai and the low β-glucan resource Chi38.The total length of the map was 2045.09 cM,and the average plot distance was 0.42 cM.The β-glucan content of RIL populations in four environments was determined by standard enzyme method and near infrared method.Combined with the determination results,QTL analysis of β-glucan content was performed by complete interval mapping method.The results showed that the β-glucan content of RIL populations was normally distributed under different environmental condi-tions,and there were superparent descendants.The coefficient of variation of β-glucan content in the four environments ranged from 9.06%to 16.63%.Seven QTLs related to β-glucan content in oat were detected by QTL mapping,distributed on 2D,3D,4C,and 4D chromosomes,with the highest contribution rate of 14.73%.The same QTL was detected in two environments with a marker interval of Chr4C_mark8361257-Chr4C_mark8384831.The results of this study provide an important theoretical basis for molecular marker-assisted breeding of oat β-glucan.
维普
万方数据