Rich germplasm resources are the basis of new varieties breeding and industrial development in proso millet.In this study,190 core germplasms of proso millet in spring sowing area of northeast China were used as the experimental materials.PCR amplification and capillary electrophoresis were performed using the previously constructed SSR markers labeled with fluo-rescence at the 5'end.According to the fragments detected by capillary electrophoresis,"0/1"was used to represent without of the fragments,and ID Analysis 4.0 was used to distinguish them.PopGene,PowerMarker,MEGA,Structure,and NTSYS were used for genetic diversity analysis.The results showed that 190 materials could be distinguished by three fluorescent SSR(RYW3,RYW6,RYW28)marker combinations.A total of 73 alleles were detected,with an average of 24.3333.The effective number of alleles(Ne)was 5.4728(RYW3)-15.8922(RYW6),with an average of 9.6469.The Shannon diversity index(I)was 2.0851(RYW3)-2.9457(RYW6),with an average of 2.4896.The observed heterozygosity(Ho)was 0.7529(RYW6)-0.9574(RYW28),with an average of 0.8876.The expected observed heterozygosity(He)was 0.8194(RYW3)-0.9398(RYW6),with an average of 0.8765.The Nei's gene diversity index(Nei)was 0.8173(RYW3)-0.9371(RYW6),with an average of 0.8741.The polymorphism information content(PIC)was 0.8656(RYW3)-0.9722(RYW6),with an average of 0.9198.Based on UPGMA,190 resources were divided into three groups.Based on the genetic structure of Structure(K=3),the core collection of proso millet was divided into three groups.The proso millet was derived from four provinces in northeast China(Heilongjiang,Jilin,Liaoning,and the part of Inner Mongolia).Based on principal component analysis,the materials were divided into four groups,which was consistent with their geographical origin.The DNA molecular identification cards of 190 core collections of northeast proso millet were constructed using online two-dimensional code technology(https://cli.im/).
关键词
糜子/东北春播区/荧光微卫星标记/毛细管电泳/DNA分子身份证
Key words
Panicum miliaceum/the northeast spring sowing area/fluorescent SSR/capillary electrophoresis/DNA molecular ID card
维普
万方数据