Calmodulin-like(CML),one of the calcium signal receptor proteins unique to plants,is involved in plant growth and development and in response to environmental signal transduction.However,the response of CML to Sugarcane mosaic virus(SCMV)infection in sugarcane(Saccharum spp.hybrid)has not been reported.In the present study,a CML gene was cloned from a Badila(S.officinarum)and designated as ScCML13.The open reading frame(ORF)of ScCML13 gene is 519 bp in length and encodes a protein with 172 aa in length.Bioinformatics analysis showed that ScCML13 was a stable hydrophilic lipoprotein in with no transmembrane domain and contains 4 Ca2+-binding EF-hand domains.Phylogenetic tree analysis showed that the ScCML13 protein differentiated in monocotyledon and dicotyledon plants and in monocotyledonous C3 and C4 plants.Yeast two-hybrid(Y2H)and bimolecular fluorescence complementation(BiFC)experiments indicated that ScCML13 interacted with P3N-PIPO,the movement protein of SCMV.Subcellular localization assays demonstrated that ScCML13 was localized to the endoplasmic reticulum and nucleus,whereas the co-localization assays showed that ScCML13 interfered with the localization of SCMV-P3N-PIPO to plasma membrane or plasmodesmata.The RT-qPCR showed that ScCML13 gene was mainly expressed in sugarcane leaves,but relatively low in internodes and roots.The ScCML13 gene was significantly up-regulated at 2 h upon SCMV infection,and then down-regulated to the levels compared with the control group,while up-regulated at the later stage of infection.
维普
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