The chloroplast marker has high genetic conservation and is not affected by nuclear gene recombination,making it suitable for the classification and management of maize germplasm resources.Based on the Maize6H-60K chip,candidate mark-ers for chloroplast grouping were obtained and 3549 maize germplasm resources were classified into five groups:B,D,H,C,and T.Twenty-nine specific chloroplast group panels(VCP)were selected and KASP primers were designed by the comparison of genotype information.A rapid group analysis algorithm compatible with chip and KASP platform was developed,which was con-sistent with the cluster analysis.Five group-specific markers were selected using sequential analysis to rapidly detect the germ-plasm resources,and a new efficient method for rapid grouping of maize germplasm resources was established,reducing the de-tection volume by 95%.This method provides a new efficient way to classify and manage maize germplasm resources.
维普
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