马铃薯TIFY基因家族的全基因组鉴定及表达分析
Genome-wide identification and expression analysis of TIFY gene family in potato(Solanum tuberosum L.)
祁稼民 1许春苗 2肖斌2
作者信息
- 1. 定西市农业综合行政执法队安定分队,甘肃定西 743000
- 2. 定西市安定区园艺工作站,甘肃定西 743000
- 折叠
摘要
TIFY家族是植物所特有的包含保守TIFY结构域(TIF[F/Y]XG)的转录因子基因家族,在调节植物发育、响应非生物胁迫和植物激素方面具有重要作用.本研究在马铃薯(Solanum tuberosum L.)全基因组水平共鉴定出了 26 个StTIFY家族成员,根据系统发育特征和蛋白结构将StTIFY分为 4 大亚家族,其中JAZ亚家族分为了 5 个亚组.串联重复和片段重复事件在马铃薯TIFY基因家族的扩张中起主要作用.通过共线性分析发现,StTIFY与双子叶植物番茄(Solanum lycopersicum)、拟南芥(Arabidopsis)、甘蓝(Brassica oleracea)分别有 21 对、5 对和 6 对直系同源基因,而和单子叶植物水稻(Oryza sativa)、玉米(Zea mays)则分别只有 1 对和 3 对直系同源基因,这些基因均在纯化选择下进化.RNA-seq数据分析发现,TIFY基因和ZML亚家族的 2 个基因在 13 个组织部位均有较高的表达量,而其余成员则呈现组织表达特异性.同时我们进一步分析了StTIFY基因对非生物胁迫以及激素处理的响应,发现有 19 个基因响应非生物胁迫(干旱/盐/热),其中分别有 3 个和 2 个基因同时响应 3 种和 2 种非生物胁迫;有 5 个基因在BAP、ABA、IAA和GA3 处理下差异表达.利用qPCR进一步检测了在干旱胁迫条件下抗旱品种和水分敏感品种中 4 个候选基因的表达量,结果发现这 4 个基因在 2 个品种的落花期显著差异表达.本研究为了解 StTIFY 基因家族的特征,深入分析StTIFY家族成员在马铃薯响应激素信号和抵御非生物胁迫的功能提供了理论依据.
Abstract
The TIFY family is a gene family of transcription factor unique to plants.It contains conserved TIFY domains(TIF[F/Y]XG),which plays an important role in regulating plant development,responding to abiotic stress,and plant hormones.In this study,a total of 26 members of the StTIFY family at the genome-wide level in potatoes(Solanum tuberosum L.)were identified.Based on phylogenetic characteristics and protein structure,StTIFY was divided into 4 subfamilies,and the JAZ subfamily was divided into 5 subgroups.Tandem duplication and segmental duplication events played a major role in the expansion of the StTIFY gene family.Collinearity analysis showed that there were 21,5,and 6 orthologous gene pairs between StTIFYs and TIFYs of dicotyledonous plants,which were tomato(Solanum lycopersicum),Arabidopsis,and cabbage(Brassica oleracea).There were only 1 and 2 orthologous gene pairs between StTIFYs and TIFYs of monocotyledonous rice(Oryza sativa)and maize(Zea mays),respectively,which had evolved under purification selection.RNA-seq data analysis showed that TIFY gene and two genes in ZML subfamily were highly expressed in 13 tissues,while the other members exhibited tissue-specificity.Furthermore,we ana-lyzed the response of the StTIFY gene to abiotic stresses and hormone treatments,and found that 19 genes responded to abiotic stress(drought/salt/heat),among which 3 genes and 2 genes simultaneously responded to 3 and 2 abiotic stresses,respectively.Five genes were differentially expressed under BAP,ABA,IAA,and GA3 treatments.The relative expression levels of four candidate genes in drought-tolerant and water-sensitive cultivars under drought stress were further detected using qPCR.The re-sults showed that these four genes were significantly differentially expressed in the two cultivars during flower-falling period.This study provides a theoretical basis for understanding the characteristics of the StTIFY gene family,as well as in-depth analysis of the functions of StTIFY family members response to hormone signals and resistance to abiotic stress.
关键词
马铃薯/TIFY基因家族/激素处理/非生物胁迫/表达分析Key words
potato/TIFY gene family/abiotic stress/hormone treatments/expression analysis引用本文复制引用
基金项目
定西市科技计划项目(DX2023BZ81)
出版年
2024
国家科技期刊平台
NSTL
万方数据