Rice(Oryza sativa L.)is a crucial cereal crop worldwide,with protein being its second-most significant nutritional component.Patients with kidney diseases are required to limit their protein intake to alleviate the metabolic burden on their kid-neys and control disease progression.In regular rice cultivars,glutelin is the predominant protein component and is easily digested by the human body.In this study,simultaneous mutations were introduced into Glutelin A1(GluA1),GluA2,and GluA3 using CRISPR/Cas9-mediated targeted mutagenesis in a japonica rice cultivar derived from Low Glutelin Content-1(LGC-1).Conse-quently,a low-glutelin rice germplasm with approximately 1.8%glutelin content,free from transgenic elements,was generated.The quality and agronomic traits of this germplasm were further comprehensively evaluated.The low-glutelin germplasms gener-ated in this study exhibited significantly lower chalkiness degree compared to the recipient cultivar,while the brown rice rate and milled rice rate were significantly higher.This study presents a highly efficient and convenient method for generating rice germ-plasm with reduced glutelin content and offers new genetic materials for the cultivation of functional rice cultivars suitable for patients with kidney diseases.
维普
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