Glutathione S-transferases(GSTs)are a class of highly conserved enzymes that play crucial roles in plant responses to environmental stresses.Bioinformatic analysis has revealed that Glycine soja GsGSTU13,which positively regulates salt-alkaline tolerance,shares the highest sequence identity with the OsGSTU17 protein.To investigate the potential contribution of GsGSTU13 to rice salt-alkaline tolerance,we transformed GsGSTU13 into rice and obtained two homozygous transgenic lines with significantly elevated GST activity.Phenotypic assays showed that after treatment with 200 mmol L-1 NaHCO3,the accumu-lation of reactive oxygen species was significantly lower in GsGSTU13 transgenic lines compared to wild-type.Additionally,the survival rates,relative water contents,and the activities of superoxide dismutases,peroxidases,catalases,and GSTs were signifi-cantly higher in GsGSTU13 transgenic lines than in the wild-type.In summary,overexpression of GsGSTU13 in rice enhanced salt-alkaline tolerance by promoting ROS scavenging,which could facilitate the breeding of new rice cultivars with improved tolerance to salt-alkaline stress.
维普
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