A total of 238 barley germplasm resources from various sources were genotyped using a high-throughput SNP chip.The β-glucan content of barley grain was determined for two consecutive years.Genome-wide association analysis was conducted using a general linear model based on the PCA model.The results showed that the β-glucan content of the 238 barley materials ranged from 1.23%to 6.55%and 1.79%to 6.64%for the respective years,exhibiting a normal distri-bution.In the GWAS analysis,a total of 19 significant SNP markers were detected,located on chromosomes 1H,2H,3H,4H,and 5H.These markers accounted for 7.39%to 10.29%of the observed phenotypic variation.Candidate genes were identified within a 300 kb range upstream and downstream of the SNP sites showing significant associations,and a total of 37 genes were identified.Through a combination of previous studies and BLAST gene annotation,four candidate genes most likely involved in β-glucan synthesis were identified.Notably,within 89 kb upstream of the most significant SNP locus B1_1033963,the candidate gene HORVU.MOREX.R3.1 HG0000140 was detected,which may play a crucial role in the process of beta-glucan synthesis.This study provides theoretical guidance and valuable gene resources for the genetic improvement of β-glucan content in barley.
关键词
大麦/β-葡聚糖/全基因组关联分析/SNP标记
Key words
barley/β-glucan/genome-wide association analysis/SNP marker
维普
万方数据