Salinity stress is a major constraint to rice production in many coastal regions,particularly in the salt-fresh water inter-face areas.Salt tolerance in rice is a complex trait that can be localized through quantitative trait loci(QTL)mapping,which can facilitate the breeding of rice varieties with enhanced salt tolerance.In this study,an F9 mapping population consisting of 174 recombinant inbred lines was developed from a cross between the coastal deep-water rice variety Chihe(donor parent)and the U.S.rice variety Lemont(receptor parent).The population was subjected to salinity stress during the germination,seedling,and reproductive stages with NaCl concentrations of 15 g L-1,5 g L-1,and 5-6 g L-1,respectively.Data were collected on relative germination rates,seedling salt tolerance grades,and seven phenotypic traits during the reproductive stages.Linkage genetic mapping and QTL mapping were performed using 142 simple sequence repeat(SSR)markers.The results indicated that Chihe was salt-sensitive at the germination stage but exhibited salt tolerance at the seedling and reproductive stages,while Lemont lines were consistently salt-sensitive.Approximately 70.11%,50.57%,and 60.34%of the lines showed salt sensitivity in the germina-tion,seedling,and reproductive stages,respectively,with a weak negative correlation of salt tolerance across the stages.A total of 33 QTLs were identified,with LOD values ranging from 2.52 to 10.32 and phenotypic variation explained ranging from 0.06%to 13.68%.Specifically,4 QTLs were identified for the germination stage,6 for the seedling stage,and 23 for the reproductive stage.Four overlapping QTLs were identified at the reproductive stage,and the QTLs contributing to the largest phenotypic variation were all derived from the salt-tolerant parent.Further investigation of these QTLs can provide new genetic resources for improv-ing salt tolerance in rice breeding,thereby aiding the development of rice varieties with enhanced tolerance to salinity stress.
关键词
全生育期/耐盐/深水稻/鉴定评价/数量性状位点
Key words
whole growth period/salt tolerance/deep-water rice/identification and evaluation/quantitative trait locus(QTL)
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基金项目
国家重点研发计划项目(2021YFD1200505-2)
国家自然科学基金项目(32260487)
国家自然科学基金项目(32360519)
广西科技重大专项(桂科AA23073004)
广西科技计划项目(桂科AB21238009)
广西农业科学院基本科研业务专项(桂农科 2021YT030)
Special Funds of Basic Scientific Research Foundation of Guangxi Academy of Agricultural Sciences(Gui Nong Ke 2021YT030)
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