The HvMBF1c gene in barley is implicated in the plant's response to salt stress.To investigate the HvMBF1c re-sponse mechanism to salt stress,we conducted bioinformatics analysis of the HvMBF1 gene,qRT-PCR analysis,and physio-logical index determination in Nakagawa barley(salt-tolerant type),GN18(salt-sensitive type),wild-type(WT)and transgenic HvMBF1c Arabidopsis thaliana treated with 200 mmol L-1 NaCl for 0,6,12,24,48,and 72 hours.The results showed that the barley HvMBF1c gene is closely related to the wheat TaMBF1c gene,and is located on chromosome 7H.The exon of HvMBF1c is larger than those of HvMBF1a and HvMBF1b,with HvMBF1a and HvMBF1b containing a typical motif 4 domain,while the HvMBF1c gene has a unique motif 5 domain.The promoter sequence of the HvMBF1c gene includes photoreactive,root-specific regulatory elements,and hypoxia-specific inducible enhancing elements,homologous to wheat chromosomes 2,3,and 7.qRT-PCR analysis revealed significant upregulation of HvMBF1c expression in two barley genotypes at the seedling,jointing,heading,and filling stages,with higher expression in Nakagawa barley compared to GN18 as salt treatment duration increased.Similarly,the expression of HvMBF1c in Arabidopsis thaliana also increased with prolonged salt treatment time.Physiological indices of Nakagawa barley at each growth stage were lower than those in GN18,and transgenic Arabidopsis exhibited lower indices compared to WT under different salt stress conditions.These findings provide a foundation for further exploration of the HvMBF1c gene function.
维普
万方数据